Fused MacroprojectilesStopping Plates

Description (Brief)
These fused macroprojectiles and stopping plates resulted from the firing mechanism for a biolistic gene gun prototype produced by John Sanford, Ed Wolf, and Nelson Allen at Cornell University in Ithaca, New York. Biolistic gene guns are used to genetically transform plants by shooting microprojectiles (tiny bullets) covered in DNA into plant cells.
The firing mechanism of the gene gun required several steps. A gunpowder charge (see object 1991.0785.03.2) or compressed air was used to accelerate a macroprojectile (see object 1991.0785.03.3), upon whose tip rested DNA-coated microprojectiles. The macroprojectile was halted upon its impact with a stopping plate (see object 1991.0785.03.4). A hole in the stopping plate was small enough to allow the microprojectiles to pass through, but large enough to halt the macroprojectile. The microprojectiles would then continue to move forward, eventually penetrating the cells to be transformed. The process is diagrammed in the Biolistic Gene Transfer Process shadow box (see object 1992.0023.01).
To learn more about biolistic gene guns, please see gene gun prototype II (object number 1991.0785.02) or gene gun prototype III (object number 1991.0785.01.1).
Location
Currently not on view
Physical Description
plastic (overall material)
Measurements
average spatial: 2.5 cm x 8.9 cm; in x 3 1/2 in
ID Number
1991.0785.03.5
catalog number
1991.0785.03.5
accession number
1991.0785
See more items in
Medicine and Science: Biological Sciences
Science & Mathematics
Biotechnology and Genetics
Biolistic Gene Guns
Data Source
National Museum of American History, Kenneth E. Behring Center

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