Fused Macroprojectiles/Stopping Plates

Description (Brief):

These fused macroprojectiles and stopping plates resulted from the firing mechanism for a biolistic gene gun prototype produced by John Sanford, Ed Wolf, and Nelson Allen at Cornell University in Ithaca, New York. Biolistic gene guns are used to genetically transform plants by shooting microprojectiles (tiny bullets) covered in DNA into plant cells.

Description (Brief)

The firing mechanism of the gene gun required several steps. A gunpowder charge (see object 1991.0785.03.2) or compressed air was used to accelerate a macroprojectile (see object 1991.0785.03.3), upon whose tip rested DNA-coated microprojectiles. The macroprojectile was halted upon its impact with a stopping plate (see object 1991.0785.03.4). A hole in the stopping plate was small enough to allow the microprojectiles to pass through, but large enough to halt the macroprojectile. The microprojectiles would then continue to move forward, eventually penetrating the cells to be transformed. The process is diagrammed in the Biolistic Gene Transfer Process shadow box (see object 1992.0023.01).

Description (Brief)

To learn more about biolistic gene guns, please see gene gun prototype II (object number 1991.0785.02) or gene gun prototype III (object number 1991.0785.01.1).

Location: Currently not on view

See more items in: Medicine and Science: Biological Sciences, Biolistic Gene Guns, Biotechnology and Genetics, Science & Mathematics


Exhibition Location:

Data Source: National Museum of American History

Id Number: 1991.0785.03.5Catalog Number: 1991.0785.03.5Accession Number: 1991.0785

Object Name: macroprojectiles/stopping plates

Physical Description: plastic (overall material)Measurements: average spatial: 2.5 cm x 8.9 cm; in x 3 1/2 in

Guid: http://n2t.net/ark:/65665/ng49ca746aa-21f5-704b-e053-15f76fa0b4fa

Record Id: nmah_1167055

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