The pharmaceutical firm, Parke, Davis & Company of Detroit, Michigan, began selling culture media for bacteriological work beginning about 1897. This box of fifty glass culture media tubes was intended for use by universities, medical colleges, boards of health, and hospitals.The set includes samples of eight different culture media: Nutrient Gelatin, Glucose Gelatin, Litmus Gelatin, Nutrient Agar, Glycerin Agar, Glucose Agar, Blood Serum according to Dr. Koch, Blood Serum - Loeffler's mixture for diptheria, and several empty tubes. The box also includes tubes containing a sterilzed cotton swab used for making throat cultures to diagnose diphtheria. All of the tubes were sterilized, plugged with cotton and rubber-capped. A box of fifty tubes cost $2.50 in 1897. This specimen was given to the Smithsonian in 1898 by the company. (See objects MG.142336.02 through MG.142336.12 for examples of the individual tubes.)
reference: Parke, Davis & Co. Price List, 1897, pp.108, 180.
An inscription on the cardboard box reads in part, “THE KEIDEL VACUUM BLEEDING TUBE / FOR THE QUICK AND ASEPTIC COLLECTION OF BLOOD FOR THE WASSERMANN AND OTHER REACTIONS.” The Wasserman test for syphilis was developed at the Robert Koch Institute for Infectious Diseases, in 1906, and named for August Paul von Wassermann (1866-1925), a German bacteriologist. Albert Keidel (1877-1942), a physician affiliated with the Johns Hopkins School of Medicine in Baltimore, designed the form of this tube.
Ref: Albert Keidel, “A Sample Bleeding Tube for Obtaining Specimens for the Wasserman Reaction,” Journal of the American Medical Association 58 (1912): 1579.
Testing blood serum for the presence of antibodies required specialized tools and techniques for collecting blood samples without introducing contaminants. The Keidel Vacuum Bleeding Tube, introduced around 1915, provided one solution. Each sterile package contained a needle attached via a short rubber tube to a sealed glass vacuum tube. After the needle was inserted into the vein, the seal was broken, allowing blood to be drawn quickly into the glass tube. The sample could then be resealed and sent to the laboratory for testing. The Keidel device was marketed particularly for the Wassermann test—a serological test for syphilis developed in 1906. The diagnostic test aided public health departments in their efforts to control the spread of sexually transmitted diseases.
Wood chest with latched, hinged lid. Paper label on top of lid. Additional paper label with list of contents of kit adhered to inside of chest lid. Chest contains: one 30 cc. bottle of Physiological Salt Solution, one 30 cc. bottle of Suspension of Sterilized Culture of Typhoid Bacillus, one 10 cc. dropping flask for Typhoid Culture with rubber bulb attached, one 10 cc. dropping flask for Salt Solution with rubber bulb attached, five graduated test tubes (one missing from original count) , one metal test tube holder (which holds six test tubes), three glass droppers (one has rubber bulb attached); one cardboard box with nine small capillary tubes for collecting serum, one needle in glass capillary tube, extra rubber bulbs and rubber stopper. Kit also contains one paper booklet with directions. One newspaper clipping - "Easy Method of Doing Widal's Reaction for Typhoid" / "Early Closure of War Wounds" - ca 1916, found in kit.
In 1896, French physician and bacteriologist Georges Fernand Isidore Widal introduced a blood test for typhoid that still bears his name. Scientists had observed that cholera bacteria would clump together when injected into animals that had been immunized against the disease. This clumping, called agglutination, resulted from the binding of antibodies in the blood serum to the bacterial antigens. The clumps were easily observed through a microscope. Widal devised a practical diagnostic technique for typhoid based on this phenomenon. He mixed a small sample of the patient’s blood serum with a suspension of typhoid bacilli, then used a microscope to examine a drop of the solution. If the cells clumped together, the patient had typhoid antibodies, which indicated either current infection or prior exposure to the disease.
In 1905, bacteriologist John Borden modified the Widal test in a way that freed practicing physicians from their reliance on the services of bacteriological laboratories. By 1912, the Mulford company was advertising a complete test outfit based on his modifications. The kit included a needle and glass capillary tubes for collecting blood from the earlobe, a bottle of salt solution for diluting the sample, a bottle of killed typhoid bacilli suspension, dropper bottles, test tubes, and a rack. The test required no microscope, as the bacterial clumping was visible to the naked eye, appearing as a small white mass at the bottom of the test tube.
This handheld device detects the presence of troponin T in blood, which is a marker for for myocardial infraction (heart attack). The device is used by health care professionals to quickly diagnosis cardiac events and to determine whether there is damage to the heart muscle.